preparation of agalactia vaccine in fermentor

Preparation of agalactia vaccine in fermentor

Mycoplasma agalactia was cultured in PPLO broth medium supplemented with sterilized normal horse serum in fermentor. Cultivation process continued at 37 ºC under aerobic conditions and growth was terminated after 26 hours cultured period with 2.2×109 Mycoplasma per ml. Samples were taken at different stages of growth. Culture was inactivated with 0.04 %v/v formaldehyde and the vaccine was adjuv...

Mycoplasma Agalacciae. V- Comparison of three different contagious agalactia vaccine

Determination of Antibody Response against Inactivated Agalactia Vaccine in Small Ruminants

  An inactivated agalactia vaccine was prepared with Mycoplasma agalactiae in fermenter. The culture was inactivated and subsequently adjuvanted with aluminum hydroxide gel. Four sheep and four goats were immunized with the experimental vaccine. Two doses of 1ml of vaccine were administered three weeks interval. Monthly, blood samples were taken from the vaccinated animals up to the fifth month...

Preparation of concentrated blackleg vaccine

  Blackleg in cattle has been recognized since 1938 in Iran that mostly affects cattle in enzootic farm. Main object of this study was to prepare and formulate a concentrated potent vaccine for immunization of cattle against blackleg in Iran. Experimental concentrated blackleg vaccine was prepared according to the method described by FAO. The medium (Modified medium for production of experiment...

Vaccine Preparation and Quality Control of Killed Leishmania major

Use of Sodium Alginate Adjuvant in Preparation of a Formalin Killed Vaccine Against Infectious Bovine Rhinotracheitis